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Constant Systems Ltd ts series benchtop cell disruptor
Ts Series Benchtop Cell Disruptor, supplied by Constant Systems Ltd, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ts+series+benchtop+cell+disruptor/cell+disruptor/pmc12656268-163-34-39
Average 86 stars, based on 1 article reviews
ts series benchtop cell disruptor - by Bioz Stars, 2026-09
86/100 stars

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Lysis:

Article Title: Rapid purification method for human SFPQ by implementing zinc-induced polymerization.
Article Snippet: Splicing factor prolineand glutamine-rich (SFPQ) is an RNA-binding protein, playing significant roles in gene regulation and subnuclear body formation.. Our recent serendipitous discovery showed that SFPQ binds zinc directly and forms an infinite polymer that is induced by zinc binding to the protein.. The zinc-induced reversible polymerization has led us to exploit this property to develop a rapid purification strategy for SFPQ without the use of affinity tags.

Article Title: Venom Peptides Across Asian and American Tarantulas Utilize Dual Pharmacology to Target Activation and Fast Inactivation of Voltage-Gated Sodium Channels
Article Snippet: The incubation temperature was then reduced to 16 °C, and protein production was induced with 0.5 mM isopropyl β-D-1-thiogalactopyranoside (IPTG; Astral Scientific, Taren Point, NSW, Australia). .. After 16 h of induction, cells were collected by centrifugation, and the resulting pellets were resuspended in TN buffer (50 mM Tris, 300 mM NaCl, 15 mM imidazole, pH 8.0) before lysis using a TS Series Benchtop Cell Disruptor (Constant Systems Ltd., Daventry, UK). .. Soluble lysate was collected by centrifuging and the fusion protein (His6-MBP-peptide) purified via nickel ion affinity chromatography using charged Ni-NTA superflow resin (QIAGEN), and the fusion protein was eluted with TN buffer containing 300 mM imidazole.

Bacteria:

Article Title: Post-translational Succinylation of Mycobacterium tuberculosis Enoyl-CoA Hydratase EchA19 Slows Catalytic Hydration of Cholesterol Catabolite 3-Oxo-chol-4,22-diene-24-oyl-CoA.
Article Snippet: Matrix-assisted laser-desorption time-of-flight (MALDI-TOF) MS was conducted on a Bruker Autoflex II TOF/TOF for MALDI-TOF. .. Bacteria cells were lysed with a Constant Systems TS series benchtop cell disruptor (Kennesaw, GA). .. Fatty acid and cholesterol substrates were purified using a Shimadzu (Somerset, NJ) HPLC system.

Starch:

Article Title: A highly efficient nanoscale tapioca starch prepared by high-speed jet for Cu 2+ removal in simulated industrial effluent.
Article Snippet: BACKGROUND: Nanoscale tapioca starch (NTS) was successfully developed by high-speed jet in our previous study.. In this study, the adsorption capacity of Cu onto NTS was further discussed.. The optimal adsorption conditions (pH, contact time, contact temperature, initial Cu concentration, and adsorbent concentration), adsorption kinetics, isotherms, and thermodynamic were also evaluated.

Centrifugation:

Article Title: Structural and Functional Investigation of the Periplasmic Arsenate-Binding Protein ArrX from Chrysiogenes arsenatis .
Article Snippet: .. The cells were lysed using a TS series benchtop cell disruptor (Constant Systems Ltd.) at 35 kpsi, and insoluble debris were removed by centrifugation (Beckman JLA-25.50, 30,000g, 20 min; 4 °C). .. The soluble fraction was incubated with Ni-Sepharose 6 Fast Flow resin (5 mL; GE Healthcare Life Sciences; 4 °C; 1 h stirring), which was pre-equilibrated with binding buffer (20 mM potassium phosphate, 500 mM NaCl, 40 mM imidazole, pH 7.2).

Article Title: Venom Peptides Across Asian and American Tarantulas Utilize Dual Pharmacology to Target Activation and Fast Inactivation of Voltage-Gated Sodium Channels
Article Snippet: The incubation temperature was then reduced to 16 °C, and protein production was induced with 0.5 mM isopropyl β-D-1-thiogalactopyranoside (IPTG; Astral Scientific, Taren Point, NSW, Australia). .. After 16 h of induction, cells were collected by centrifugation, and the resulting pellets were resuspended in TN buffer (50 mM Tris, 300 mM NaCl, 15 mM imidazole, pH 8.0) before lysis using a TS Series Benchtop Cell Disruptor (Constant Systems Ltd., Daventry, UK). .. Soluble lysate was collected by centrifuging and the fusion protein (His6-MBP-peptide) purified via nickel ion affinity chromatography using charged Ni-NTA superflow resin (QIAGEN), and the fusion protein was eluted with TN buffer containing 300 mM imidazole.

Cell Culture:

Article Title: The crystal structures of a copper-bound metallochaperone from Saccharomyces cerevisiae.
Article Snippet: Atx1 is a metallochaperone protein from the yeast Saccharomyces cerevisiae (yAtx1) that plays a major role in copper homeostasis in this organism. yAtx1 functions as a copper transfer protein by shuttling copper to the secretory pathway to control intracellular copper levels.. Here we describe the first crystal structures of yAtx1 that have been determined in the presence of Cu(I).. The structures from two different crystal forms have been solved and refined to resolutions of 1.65 and 1.93 Å.

Concentration Assay:

Article Title: The crystal structures of a copper-bound metallochaperone from Saccharomyces cerevisiae.
Article Snippet: Atx1 is a metallochaperone protein from the yeast Saccharomyces cerevisiae (yAtx1) that plays a major role in copper homeostasis in this organism. yAtx1 functions as a copper transfer protein by shuttling copper to the secretory pathway to control intracellular copper levels.. Here we describe the first crystal structures of yAtx1 that have been determined in the presence of Cu(I).. The structures from two different crystal forms have been solved and refined to resolutions of 1.65 and 1.93 Å.

Incubation:

Article Title: The crystal structures of a copper-bound metallochaperone from Saccharomyces cerevisiae.
Article Snippet: Atx1 is a metallochaperone protein from the yeast Saccharomyces cerevisiae (yAtx1) that plays a major role in copper homeostasis in this organism. yAtx1 functions as a copper transfer protein by shuttling copper to the secretory pathway to control intracellular copper levels.. Here we describe the first crystal structures of yAtx1 that have been determined in the presence of Cu(I).. The structures from two different crystal forms have been solved and refined to resolutions of 1.65 and 1.93 Å.



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